芽孢杆菌类益生素对肉鸡血细胞及免疫器官组织结构的影响

doi:10.11843/j.issn.0366-6964.2013.05.016

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (5): 778-787.doi: 10.11843/j.issn.0366-6964.2013.05.016

芽孢杆菌类益生素对肉鸡血细胞及免疫器官组织结构的影响

靳二辉¹，陈耀星²，王群³，乔恩美¹，吴国忠⁴，李升和^1*

（1. 安徽科技学院动物科学学院，凤阳 233100；2.中国农业大学动物医学院，北京 100193；3. 浙江省嘉善县畜牧兽医局，嘉善 314100；4.中国科学院上海应用物理研究所，上海 201800）

收稿日期:2012-10-08 出版日期:2013-05-23 发布日期:2013-05-23
通讯作者: 李升和（1974-），男，安徽定远人，博士，教授，主要从事动物免疫学、动物组织病理学及微量元素营养与健康研究，E-mail：lishhe2005@126.com
作者简介:靳二辉（1980-），男，河南汝州人，博士，讲师，主要从事生殖免疫与微量元素营养研究，E-mail:erhuijin2011@126.com
基金资助:
国家星火计划面上项目（2012GA710027）；安徽省自然科学基金（1208085QC68）；安徽省农业科技成果转化资金项目（1104b0303013；12040302012）；安徽省高校自然科学研究项目（KJ2012B057）；安徽科技学院重点学科基金项目（AKXK20101-2）

Effect of the Bacillus Probiotics on Blood Cells and Structure of Immune Organs in Broilers

JIN Er-hui¹, CHEN Yao-xing², WANG Qun³, QIAO En-mei¹, WU Guo-zhong⁴, LI Sheng-he^1*

(1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100,China; 2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;3. Animal Husbandry and Veterinary Bureau of Jiashan County of Zhejiang, Jiashan 314100, China;4. Shanghai Institute of Applied Physics, Chinese Academy of Science, Shanghai 201800, China)

Received:2012-10-08 Online:2013-05-23 Published:2013-05-23

摘要/Abstract

摘要：

为了研究芽孢杆菌类益生素对肉鸡血细胞和免疫器官组织结构的影响，本研究选用480羽刚出壳AA肉鸡，随机分对照组、抗生素组、试验Ⅰ和Ⅱ组，对照组饲喂基础日粮，抗生素组添加50 mg·kg^－1金霉素，试验Ⅰ和Ⅱ组分别添加200和400 mg·kg^－1富含芽孢杆菌类益生素，试验期42 d。结果显示，与对照组相比，试验Ⅱ组第21和42天肉鸡红细胞数量、嗜碱性粒细胞和单核细胞比例分别显著或极显著增加（P<0.05或P<0.01），第42天胸腺、法氏囊重量和器官指数分别显著增加46.60%和38.23%、66.67%和47.62%（P<0.05）；试验Ⅰ组第21和42天肉鸡法氏囊重量和器官指数分别显著增加35.40%和36.92%、88.03%和153.97%（P<0.05），第42天胸腺重量和器官指数显著增加46.27%和28.13%（P<0.05）；抗生素组白细胞数量显著减少，第42天脾脏重量和器官指数显著降低（P<0.05）。试验Ⅰ和Ⅱ组第21和42天肉鸡免疫器官与对照组相比，脾小结数量增多、脾动脉周围淋巴鞘增厚，胸腺皮质增厚、胸腺小体数量减少，法氏囊小结数量增多、小结内淋巴细胞排列紧密，且试验Ⅰ组比试验Ⅱ组更为明显，而抗生素组免疫器官均出现不同程度的发育不良。上述结果表明，添加200 mg·kg^－1芽孢杆菌益生素比400 mg·kg^－1更能促进免疫器官的发育，提示芽孢杆菌类益生素具有增强机体免疫功能的作用。

Abstract:

In order to study the effect of the Bacillus probiotics on blood cells and tissue structure of broilers, 480 newly hatched AA chicken were randomly divided into control group, antibiotic group, experiment Ⅰ and Ⅱ groups. The control group was fed with basal diet, and the antibiotic group was fed with 50 mg·kg^－1 chlortetracycline in the basal diet, and the experimental Ⅰ and Ⅱ groups were respectively fed with 200 and 400 mg·kg^－1 the Bacillus probiotics in the basal diet for 42 days. The results showed: compared with the control group, the quantity of red cells and the ratio of basophilic granulocytes and monocytes were significantly increased in 21- and 42-day- old broilers (P<0.05), and the weight and organ index of thymus and bursal of the 42-day-old broilers were respectively increased by 46.60% and 38.23%, 66.67% and 47.62% (P<0.05); The weight and organ index of bursal were significantly increased by 35.40% and 36.92%, 88.03% and 153.97% in the 21- and 42-day-old broilers (P<0.05), and the weight and organ index of thymus of the 42-day-old broilers were significantly increased by 46.27% and 28.13% in experiment Ⅰ group (P<0.05); the quantity of white cells was obviously decreased, and the weight and organ index of spleen were also obviously decreased in the antibiotic group at the 42 d (P<0.05). Compared the tissue structure of immune organs in experiment Ⅰand Ⅱ group with the control group, the quantity of spleen node were increased, and periarterial lymphatic sheath were thickened; the thymic cortex were also thickened, and the quantity of thymic corpuscle were decreased; the bursal node were greatened with tightly rank lymphocytes, and which change were more obvious in the experiment Ⅰthan the experiment Ⅱ group. But the tissue structure of immune organs showed different degree atrophy in the antibiotic group. The results suggested that addition of 200 mg·kg^－1 the Bacillus probiotics are better to improve development of immune organs and strengthen immune function than that of 400 mg·kg^－1 the Bacillus probiotics.

中图分类号:

靳二辉，陈耀星，王群，乔恩美，吴国忠，李升和. 芽孢杆菌类益生素对肉鸡血细胞及免疫器官组织结构的影响[J]. 畜牧兽医学报, 2013, 44(5): 778-787.

JIN Er-hui, CHEN Yao-xing, WANG Qun, QIAO En-mei, WU Guo-zhong, LI Sheng-he. Effect of the Bacillus Probiotics on Blood Cells and Structure of Immune Organs in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 778-787.

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芽孢杆菌类益生素对肉鸡血细胞及免疫器官组织结构的影响

Effect of the Bacillus Probiotics on Blood Cells and Structure of Immune Organs in Broilers

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